For a given rice variety, its tillering ability is mainly determined by its genetic background although it could be affected by environmental conditions such as light, temperature, plant density, nutrient and water supply. A famous example was the “New Plant Type” rice varieties with low tiller number and large panicles, which were developed by the International Rice Research Institute. For a long time, desirable plant architecture which focused on tiller number has been the main goal for breeders to breed rice varieties with high-yield potential. Tiller number is considered to be one of the key factors that affects rice plant architecture and grain yield because of its involvement in determining the final number of productive panicles. Introduction Rice is one of the most important cereal crops for more than half of the world’s population. The informative SNP marker cd-733C/T will facilitate marker-assisted selection of ts1 in rice plant type breeding. The results above provide a basis for further functional characterization of ts1 and will shed light on molecular mechanism of rice tillering. Our data indicated that ORF4 was a strong candidate gene for ts1 and ts1 might play a role in regulating rice tillering through MOC1 and HTD1 associated pathway. Quantitative RT-PCR analysis showed that the expression level of ORF4 in ts1 plants was significantly lower than that in their wild plants, and the expression of rice tillering regulators MOC1 and HTD1 was also significantly decreased in ts1 plants. ![]() A co-dominant SNP marker cd733C/T was subsequently developed and the SNP assay demonstrated that the point mutation co-segregated with tiller suppression phenotype. Among them, ORF4 (LOC_Os02g01610) is a PPR gene which harbored a point mutation c.+733/C!T in ts1 mutant plants. Candidate gene analysis identified nineteen putative genes. With an F2 population derived from a cross between ts1 and an indica cultivar Wushansimiao, a major gene, tiller suppression 1 (ts1) was fine-mapped to a 108.5 kb genomic region between markers ID8378 and SSR6884 on the short arm of rice chromosome 2. Morphological and histological observations revealed that, in the ts1 plants, the tiller buds were abnormally formed and therefore cannot outgrow into tillers. In this study, an EMS-induced rice tiller suppression mutant ts1 was characterized. Competing Interests: The authors have declared that no competing interests exist.Ībstract Tiller number is one of the key factors that influences rice plant type and yield components. The funders had no roles in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ![]() Funding: This research was supported by grants from the Special Transgenic Program of Chinese Ministry of Agriculture (No. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ![]() pone.0170574 Editor: Frank Alexander Feltus, Clemson University, UNITED STATES Received: DecemAccepted: JanuPublished: JanuCopyright: © 2017 Liu et al. OPEN ACCESS Citation: Liu L, Meng F, He Y, Zhu M, Shen Y, Zhang Z (2017) Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice. Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice Lei Liu, Fen Meng, Yonggang He, Menghao Zhu, Yanhao Shen, Zhihong Zhang* State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China * Ī1111111111 a1111111111 a1111111111 a1111111111 a1111111111
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